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KMID : 1171820070220010075
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Journal of Environmental and Sanitary Engineering
2007 Volume.22 No. 1 p.75 ~ p.83
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Clove Extracts on Tyrosinase Gene Expression
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Chin Jong-Eon
Lee Jeong-Yong
Kim Kwan-Chun
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Abstract
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Clove extract by methanol increased expression of the tyrosinase gene on B16 mouse melanoma cells containing tyrosinase promoter. 10 ㎍/mL and 100 ㎍/mL of the extract showed expression rate of the tyrosinase gene about 138% and 245%, respectively, compared with control. At 500 ㎍/mL, expression rate of the extract was impossible to measurement by high cytotoxicity. The solvent fraction of methylene chloride also exhibited highly expression rate as methanol extract. However, the solvent fractions of butyl alcohol and water showed repressive effect on expression of tyrosinase gene at 500 ㎍/mL. In MTT assay, cell survival rate of the extract exhibited similar to expression rate of tyrosinase gene. That is, 10 ㎍/mL and 100 ㎍/mL of the extract showed the cell survival rate about 128% and 187%, respectively.
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KEYWORD
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clove, b16 mouse melanoma cell, tyrosinase gene, expression
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